毛橘红总黄酮对酒精性肝损伤大鼠肝组织病理的影响

Effect of Total Flavonoids of Citrus grandis Osbeck.Var.tomentosa Hort.on Hepatic Pathological Histology of Alcoholic Liver Injury Rats

目的探讨毛橘红总黄酮在组织及细胞水平对酒精性肝损伤模型的影响。方法将雄性大鼠随机分为正常对照组、模型组、五子衍宗丸组(阳性药对照)及毛橘红总黄酮高、中、低剂量组,采用白酒灌胃造成酒精性肝损伤模型,造模同时进行药物治疗,28 d后取各组大鼠肝组织,常规HE染色,光镜观察。用乙醇体外诱导L-02肝细胞损伤,分别加入不同浓度的毛橘红总黄酮作用于L-02酒精性肝损伤细胞,检测培养液中丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)活性,MTT法检测肝细胞存活率。结果模型组肝呈典型酒精肝组织病变特征,毛橘红总黄酮各剂量组脂变程度、水肿程度以及炎性程度均有不同程度减轻;毛橘红总黄酮可显著降低L-02酒精性肝损伤细胞模型中的ALT、AST含量,增加细胞存活率。结论毛橘红总黄酮对大鼠酒精性肝病理损伤及L-02酒精性肝损伤细胞有一定保护作用。

Objective To observe the effect of total flavonoids of Citrus grandis Osbeck. Var. tomentosa Hort. （CGO Var. TH） on hepatic pathological histology in alcoholic liver injury rats. Methods Male rats were randomized into normal group, model group, positive control group （treated with Wuzi Yanzong Pills）, and high-, middle- and low-dosage Citrus grandis total flavonoids groups. We established the rat model of alcoholic liver injury by gastric gavage of alcohol, and gave the corresponding medication at the same time. After treatment for 28 days, the rats were executed and the histological features of rat liver were observed after HE staining under optical microscope. Injured L-02 hepatocytes were induced by alcohol, and then were cultured together with different concentrations of Citrus grandis total flavonids. After culturing, the activities of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in the culture so lution were measured, and the survival rate of L-02 hepatocytes was detected by MT＇F. Results In the model group, the typical hepatic pathological features of alcoholic liver injury were shown, and then the pathological changes such as fatty degeneration, edema and infammation were relieved to various degrees in Citrus grandis total flavonoids groups. Citrus grandis total flavonoids showed obvious effect on decreasing ALT and AST activities of in-vitro injured L-02 hepatocyter, and the survival rate of L-02 hepatocytes was increased. Conclusion The total flavonoids of Citrus grandis Osbeck. Var. tomentosa Hort. has certain protective effect on alcoholic liver injury rats.