龙葵的质量分析研究

Quality Analysis of Solanum nigrum by Ultra-performance Liquid Chromatography

目的建立澳洲茄碱、澳洲茄边碱和khasianine的UPLC同时检测方法,为龙葵药材质量评价提供方法。方法采用UPLC法,ACQUITY UPLC BEH C18色谱柱(2.1 mm×250 mm,1.7μm),甲醇-0.5%乙酸水溶液为流动相,梯度洗脱,检测波长为205 nm,流速为0.5 mL/min,柱温为35℃。结果龙葵药材中3个成分进样量依次在0.865～17.300μg、0.730～14.600μg,0.700～14.000μg内与峰面积线性关系良好(r=0.9999),平均加样回收率(n=9)分别为98.86%、98.91%、98.27%,RSD值均小于1.50%;广东省境内8个产区龙葵药材质量存在差异。结论该方法快速简便、准确可靠、灵敏度高、重复性好,可为龙葵药材的质量控制提供快速准确的检测方法。

Objective To establish a simultaneous detection method for solasonine, solamargine and khasianine in the medicinal material of Solanum nigrum by ultra-performance liquid chromatography（UPLC）, and to analyze the quality of the medicinal material. Methods UPLC was used to determine the contents of three components in Solanum nigrum. The samples were separated on ACQUITY UPLC BEH Cls column（250 mm × 2.1 mm, 1.7 μm） with methanol（A） - 0.5 % acetic acid （B） as the mobile phase and by the gradient elntion process. The flow rate was 0.5mL· min-1, the column temperature was set at 35 ℃, and the detection wavelength was 205 nm. Results The linear ranges of solasonine, solamargine and khasianine in Solanum nigrum were 0.865 - 17.300, 0.730 - 14.600, 0.700 - 14.000 μ g （r=0.9999）, and their average recoveries （n=9） were 98.86 %, 98.91%, 98.27 %, respectively. All of their RSD values were less thanl.50 %. The results showed that there had certain difference of quality of the medicinal material from 8 habitats of Guangdong province. Conclusion The method is rapid, convenient, stable, accurate, reliable and sensitive, which can be used for the quality control of Solanum nigrum.