栽培掌叶大黄不同部位中蒽醌类衍生物的含量分析

Content Analysis of Anthraquinones from Different Medicinal Parts of Cultivated Rheum palmatum L.

目的测定栽培掌叶大黄药材不同部位中游离蒽醌、总蒽醌及结合蒽醌的含量,为其药材质量评价和资源合理利用提供科学依据。方法采用Agela Venusil XBP-C18柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液(75∶25)为流动相,流速为1.0 mL.min-1,检测波长为254 nm,柱温为35℃。结果栽培掌叶大黄不同部位均含有5种蒽醌类衍生物且含量差异较大,主要分布在根、根茎、须根、叶片、种子中,在须根和根中的含量远高于其他部位。须根中游离蒽醌总量高于其他部位,游离蒽醌总量地下部分远高于地上部分;结合蒽醌总量则叶片中最高,结合蒽醌总量地上部分高于地下部分;蒽醌总量地下部分与地上部分相差不大,地下部分略高于地上部分。结论在实际应用时根据不同需要选择不同的药用部位。

Objective To determine the content of free anthraquinons, total anthraquinons and combined anthraquinons from different medicinal parts of Rheum palmatum L., and to provide scientific basis for quality assessment and reasonable utilization. Methods Agela Venusil XBP-C18（4.6mm×250mm,5μm） was used with methanol-0.1% phosphoric acid solution（75 ： 25） as the mobile phase at the flow rate of 1.0 mL·min-1, the wavelength was set at 254 nm, and the column temperature was set at 35 ℃. Results Five kinds of anthraquinons were all found in different medicinal parts of R. Palmatum L., but their content varied. They were mainly distributed in the root, rhizome, fibrous root, leaves and seeds. The content was much higher in the root and rhizome than that in the other medicinal parts. The content of total free anthraquinon was the highest in fibrous root, and was higher in underground parts than that in aerial parts. The content of combined anthraquinon was the highest in leaves, and was higher in aerial parts than that in underground pans. The content of total anthraquinone from underground parts was slightly higher than that from the aerial pans. Conclusion The application of the medicinal parts of Rheum palmatum L. should be based on the practical needs.