摘要
目的建立党参中管花党参碱A的HPLC含量测定方法。方法色谱柱为Kromasil C18（4．6mm×250mm，5μm）；流动相为乙腈-0．1％二乙胺（10：90）等度洗脱；流速1．0mL／min；柱温30oC；检测波长为267nm。结果管花党参碱A在0．658～6．578μg范围内线性关系良好，r为0．9990；平均回收率为96．04％，RSD为2．04％。结论该方法准确可靠，重现性好，可用于党参药材的质量控制。
Objective To develop a method for determining codotubulosine A in Radix Codonopsis by HPLC. Methods HPLC was carried out on Kromasil Cls column（4.6 mm x 250 mm, 5 μm） at the temperature of 30℃. The mobile phase consisted of CH3CN -0.1% diethylamine （10 ： 90） with gradient elution, UV detection wavelength was set at 267 nm, and the flow rate was 1.0 ml/min. Results The linear range of codotubulosine A was 0.658-6.578 μg, with the correlation coefficient being 0.9990. The average recovery was 96.04 % and the RSD was 2.04 %. Conclusion The method is accurate and reliable, which can be used to control the quality of Radix Codonopsis.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第6期678-680,683,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
