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Dynamic Changes of Nitrate Reductase Activity within 24 Hours

硝酸还原酶活性昼夜变化的研究(英文)
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摘要 [Objective] The research aimed to study the circadian rhythm of nitrate re- ductase activity (NRA) in plant. [Method] The wheat plants at heading stage were used as the materials for the measurement of dynamic changes of nitrate reductase activity (NRA) within 24 h under the conditions of constant high temperature. [Resulti The fluctuation of NRA in wheat changed greatly from 20:00 pm to 11:00 am. The enzyme activity remained constant, but at 14:00 the enzyme activity was the high- est, higher than all the other time points except the enzyme activity measured at11:00. The enzyme activity was the lowest of 17:00, which was lower than all the other time points except the enzyme activity measured at 2:00. [Conclusion] There were autonomous rhythm changes of NRA in wheat in a certain degree. [目的]为探讨植物硝酸还原酶活性的昼夜节律性。[方法]以Hoagland营养液栽培的抽穗期小麦为材料,在保持光、温恒定条件下,24h内采用活体法动态测定植株硝酸还原酶活性变化。[结果]小麦NRA在24h内的变化波动较大,从前一天20:00时开始到第2天上午11:00,酶活性没有显著差异;而14:00酶活性达到最高,且在0.05水平显著高于除11:00以外的其他时间点所测定的酶活性;下午17:00酶活性最低,在0.05水平显著低于除凌晨2:00意外的其他时间点所测定的酶活性。[结论]小麦NRA存在一定程度的自主节律性变化。
作者 杜瑛 王子龙
出处 《Agricultural Science & Technology》 CAS 2012年第11期2284-2286,共3页 农业科学与技术(英文版)
基金 Supported by the National Natural Science Foundation of China(31160254)~~
关键词 WHEAT 24 h Nitrate reductase ACTIVITY Solution culture 硝酸还原酶活性 动态变化 小时 小麦植株 NRA 昼夜节律 持续高温 时间点
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