RP-HPLC法同时测定复方脂质体中阿霉素与槲皮素的含量

Simultaneous Determination of Doxorubicin and Quercetin in Compound Liposome by RP-HPLC

目的:建立RP-HPLC方法,同时测定复方脂质体中阿霉素(DOX)与槲皮素(QUE)的含量。方法:Kromasil C18柱(250mm×4.6mm,5μm),甲醇-乙腈-pH 3.8磷酸缓冲盐(19∶29∶52)等度洗脱,流速1mL/min,检测波长254nm,柱温25℃。结果:DOX与QUE分别在0.50～10.00μg/mL、0.03～2.00μg/mL范围内线性良好(r分别为0.999 7、0.999 4);低、中、高浓度回收率分别为(99.53±2.56)%、(103.12±0.37)%、(103.21±2.02)%和(96.10±1.38)%、(102.30±1.76)%、(103.78±1.06)%(n=3)。结论:本方法准确,重复性好,可用于同时测定阿霉素-槲皮素复方脂质体中两种药物的含量。

Objective ： To establish a method for the simultaneous determination of doxorubicin and quercetin in Doxorubi- cin- Quercetin Compound Liposomes by RP - HPLC. Methods： The contents of doxorubicin and quercetin in Doxorubicin -Quercetin Compound Liposomes were determined by RP - HPLC. The determination was performed on Kromasil Cls （250mm ×4.6mm,Slxm） column with mobile phase composed of methanol - acetonitrile - PBS （pH 3.8 ） （19： 29： 52） at the flow rate of 1.0mL . min^-1. The detection wavelength was set at 254 nm, column temperature was 25℃ and injection volume was 30μL. Results： The linear ranges of DOX and QUE were 0. 5 - 1.0l.Lg . mL-1 （r = 0. 999 7） and 0. 03 -2. 0μ g . mL-1 （r =0. 999 4）, and the average recoveries of DOX and QUE in three concentration levels were （99.53 ± 2.56 ） %, （ 103.12 ± 0. 37 ） %, （ 103.21 ± 2.02 ） % and （ 96. 10 ± 1.38 ） %, （ 102. 30 ± 1.76 ） %, （103.78 ± 1.06）% （n = 3 ）. Conclusion：The method is accurate, rapid and reproducible for the concentration determi- nation of doxorubicin and quercetin in Compound Liposome at the same time.