大豆异黄酮对膳食诱导型代谢综合征大鼠糖脂代谢、血清瘦素及FFA的影响

Effects of Soybean Isoflavones on Sugar-lipid Metabolism,Serum Leptin and Free Fatty Acid Concentrations of Diet-induced Insulin Resistance in Rats with Metabolic Syndrome

目的:通过实验研究观察大豆异黄酮对代谢综合征大鼠胰岛素抵抗,血脂,血清瘦素及FFA的影响。方法:采用高脂高糖高盐饲料喂养诱导建立大鼠MS模型。将成模的MS大鼠随机分为模型组,大豆异黄酮高、低剂量组及西药组。灌胃4周,采用试纸法和生化法分别测血糖血脂,放免法测胰岛素及血清瘦素水平,比色法测FFA水平。结果:SIF高剂量组大鼠FBG、FINS、HOMA-IR与模型组相比均降低(P<0.05),与西药组疗效相似。与模型组相比,SIF高剂量组血清TC、TG以及LDL-C水平显著降低(P<0.01)。而SI高剂量组瘦素及FFA水平较模型组明显降低(P<0.05),与西药组无明显差异。结论:大豆异黄酮可以改善MS模型大鼠胰岛素抵抗,调节血脂,其可能与降低脂肪细胞因子瘦素及FFA水平相关。

Objective ：To observe the effect of soybean isoflavones on insulin resistance, blood lipid level, Serum Leptin and Free Fatty Acid in metabolic syndrome rats. Method： MS rat model was induced by high fat sugar and high salt diet. Then these metabolic syndrome rats were randomly divided into MS model group, Metformin group and two SI -trea- ted groups（ SIH ,SIL, ）. Blood lipid level and blood glucose were measured before and after drug intervention. Radioim- munoassay was used to detect Serum Leptin and insulin. Colorimetry was used to measure Free Fatty Acid. Results：The level of blood glucose, Serum Leptin and insulin sensitivity index in high dose SI2 treatment group were lower than those in model group （P 〈 0. 05 ）, which were similar to Metformin group. In high dose SI2 treatment group, the low density li- pop rotein cholesterol （LDLC） , triglyeeride （TG）and total cholesterol （TC）levels were significantly lower than those in model group （ P 〈 0.01 ）. Compared with model group, Serum Leptin and Free Fatty Acid levels were obviously lower （P 〈0.05 ） ,which had no remarkable difference with Metformin group. Conclusion ：SI can regulate the blood lipid level in metabolic syndrome rats, reducing the level of Serum Leptin and Free Fatty Acid.