番酯素诱导人肺癌A549细胞凋亡及其分子机制的实验研究

Experimental study on apoptosis induced by annonaceous acetogenins chinensis in human pulmonary carcinoma cell A549 and its molecular mechanisms

目的研究番酯素抑制人肺腺癌细胞A549增殖和诱导凋亡的作用及其机制。方法①MTT法观察番酯素对人肺腺癌A549细胞株、人肺腺癌NCI-H460细胞株体外增殖的影响;②流式细胞仪检测番酯素对细胞凋亡的影响。结果番酯素在体外对人肺腺癌细胞A549有一定的抑制作用,且这种抑制作用具有量-效关系,剂量越大,抑制作用越强;细胞经过番酯素处理72 h后,与对照组比较,番酯素各剂量线细胞均出现不同程度的凋亡,且总凋亡随着剂量的增加而增加。结论番酯素具有抑制人肺腺癌细胞A549增殖的作用,可以诱导其凋亡。

Objective It is to investigate the proliferation inhibiting and apoptosis inducing effects of annonaceous aceto- genins chinensis in human lung cancer cell A549 and its molecular mechanisms. Methods MTT assay was used to observe the proliferation of human pulmonary carcinoma cell A549 and human pulmonary carcinoma cell NCI - H460 in vitro. Flow cytometry was used to observe the apoptosis induced by annonaceous acetogenins. Results Annonaceous acetogenins had an inhibiting effect on the proliferation of human lung cancer cell line A549, and the inhibition was dose-dependent. When the dosage increased, the inhibition got more obviously. After cultivated with annonaceous acetogenins for 72h, compared with that in control groups, more or less apoptosis of the cells in every annonaceous acetogenins group was found. The total apoptosis rate increased when the dosage increased. Conclusion Annonaceous acetogenins can inhibit human lung cancer cell line A549 cell proliferation, and can induce its apoptosis.