低剂量亚硝酸钠对失血性休克大鼠肠道炎症反应的影响

目的探讨应用低剂量亚硝酸钠对失血性休克后大鼠肠道炎症反应的影响。方法清洁级健康雄性SD大鼠30只,随机分为3组(n=10):假手术组(S组)、失血性休克组(HS组)、亚硝酸钠组(NI组)。HS组采用颈动脉放血制做模型,休克持续1h后回输自体血及生理盐水复苏,NI组于复苏后10min给予亚硝酸钠干预,复苏2h后,取小肠组织行常规病理学检查,免疫组织化学方法测定其肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)并测定髓过氧化物酶(mye loperoxidase,MPO)。结果与S组相比,HS组,NI组肠黏膜结构破坏,TNF-α和MPO水平增加;与HS组相比,NI组肠粘膜损伤程度轻于HS组,差异具有统计学意义(P<0.05)。结论低剂量亚硝酸钠可有效抑制肠道炎症反应,从而对失血性休克大鼠产生保护作用。

Objective To investigate the effects of low does sodium nitrite on intestinal inflammatory response of rats after hemorrhagic shock. Methods Thirty rats were randomly divided into three groups：sham operation groups, hemorrhagic shock group, sodium nitrite group. The carotid artery wag cannulated for blood-letting to establish the HS model.The shock was maintained for 1 hours until the lossed blood was replenished with amount of autoblood and physiological saline. A dose of sodium nitrite was given when medicine treatment group began to be resuscitate after 10min. After 2 hours, the content of TNF-α in the intestinal was determined and the pathological changes of intestine were observed. At the same time, the levels of MPO in intestine homogenates were determined. Results Compared with sham operation group, TNF-α and MPO were significantly increased in hemorrhagic shock group. As compared to hemorrhagic shock group, these levels were lower in medicine treatment group. The histopathologic changes were consistent with the biochemical markers. Conclusion A low does sodium nitrite could inhibit the increase in intestinal inflammatory response, and have potential pretective effects on the intestinal after hemorrhagic shock.