正交试验优选桂花籽中原花青素的提取工艺

Optimization of Extraction Technology for Procyanidins from Seed of Osmanthus fragrans by Orthogonal Test

目的:优选桂花籽中原花青素的超声提取工艺。方法:以原花青素得率为指标,选取提取时间、提取温度、料液比、乙醇体积分数为考察因素,在单因素试验基础上,采用正交试验优选超声提取工艺。结果:最佳提取工艺为加8倍量50%乙醇于50℃提取25 min。结论:优选的提取工艺快速、简单,为桂花籽的开发利用提供试验依据。

Objective： To optimize ultrasonic extraction technology of procyanidins from seed of Osmanthus fragrans. Method： With yield of procyanidins as index, extraction time, extraction temperature, solid-liquid ratio and the concentration of ethanol were chosen as factors, based on single factor test, ultrasonic extraction technology was optimized by orthogonal test. Result： Optimum extraction technology was： extracted 25 min with 8 times the amount of 50% ethanol at 50 ℃. Conclusion： This optimized extraction process was quick and simple, and it could provide experimental basis for development and utilization of seed of O. fragrans.