柚寄生总黄酮的提取工艺优化及其体外抑制人肝癌细胞Hep3B作用的初步评价

Optimization of Extraction Process and Preliminary Evaluation of in vitro Inhibitory Activity on Human Hepatoma Cell Hep3B of Total Flavonoids from Viscum ovalifolium

目的:优选柚寄生总黄酮的提取工艺,并初步评价总黄酮体外抑制人肝癌细胞Hep3B的作用。方法:以芦丁为对照品测定柚寄生提取液的总黄酮含量,选取提取时间、提取温度、料液比及乙醇体积分数为影响因素,采用正交试验法优选柚寄生总黄酮的提取工艺条件,采用单因素试验考察提取次数;采用MTT法测定总黄酮对人肝癌细胞Hep3B的体外抑制作用。结果:优选的提取工艺为加30倍量60%乙醇于80℃提取2次,每次2 h;体外抗肿瘤活性试验结果表明,总黄酮质量浓度500 mg·L-1时对人肝癌细胞Hep3B的生长抑制率51.40%。结论:优选出的柚寄生总黄酮提取工艺条件稳定可行,柚寄生总黄酮有一定的体外抑制人肝癌细胞Hep3B的作用。

Objective： To optimize extraction process of total flavonoids from Viscum ovalifolium, and to preliminary evaluate in vitro inhibitory effect of total flavonoids on human hepatoma cell Hep3 B. Method： With rutin as standard substance, the content of total flavonoids was determined by UV, orthogonal test was used to optimize extraction technology of total flavonoid from V. ovalifolium with extraction time, extraction temperature, solid-liquid ratio and the concentration of ethanol as factors, extraction times was investigated by single factor test; In vitro inhibition effect of total flavonoids on Hep3B was measured by MTT method. Result： Optimum extraction technology was ： extracted 2 times with 30 times the amount of 60% ethanol at 80 ℃ , 2 h per time ; In vitro antitumor activity screening showed, when treated with the concentration of total flavonoid 500 mg · L^-1, rate of growth inhibition on Hep3B was 51.40%. Conclusion： Optimized extraction conditions was stable and feasible, total flavonoids from V. ovalifolium showed some in vitro inhibitory effect on Hep3 B.