正交试验法优选中风活心软胶囊提取工艺

Optimization of Extraction Technology for Zhongfeng Huoxin Soft Capsule by Orthogonal Test

目的:优选中风活心软胶囊的提取工艺。方法:采用水蒸气蒸馏法提取中风活心方中挥发油,以挥发油得率为指标,选取加水量、浸泡时间及蒸馏时间为考察因素,通过正交试验优选挥发油提取工艺;以葛根素含量为指标,选取加水量、煎煮时间及提取次数为考察因素,通过正交试验优化中风活心软胶囊的提取工艺。结果:最佳挥发油提取工艺为加6倍量水,不浸泡,蒸馏提取7 h;中风活心软胶囊的最佳水提取工艺为加10倍量水煎煮1.0 h,提取3次。结论:优选的提取工艺稳定可行,可推广于大生产应用。

Objective： To optimize extraction technology of Zhongfeng Huoxin soft capsule. Method： Volatile oil from Zhongfeng Huoxin prescription was extracted by steam distillation method, with yield of volatile oil as index, orthogonal test was adopted to optimize extraction technology with the amount of water, soaking time and distillation time as factors ; With the content of puerarin as index, the amount of water, boiling time and extraction times were selected as factors, extraction technology of Zhongfeng Huoxin soft capsule was optimized by orthogonal test. Result： The best extraction technology of volatile oil was as follows： without soaking, distilled 7 h with 6 times the amount of water; optimum water extraction process of Zhongfeng Huoxin soft capsule was as follows： extracted 3 times with 10 times the amount of water, 1.0 h each time. Conclusion： This optimized extraction technology was stable and reliable, it could be extended to large-scale production application of Zhongfeng Huoxin soft capsule.