维药刺山柑果实中香草酸和原儿茶酸的含量测定

Determination of Vanillic Acid and Protocatechuic Acid in Cappers spinoza by HPLC

目的:建立同时测定刺山柑果实中香草酸和原儿茶酸含量的高效液相色谱法。方法:采用Dikma Kromasil C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.1%磷酸水梯度洗脱,紫外检测波长258 nm,流速0.8 mL·min-1。结果:刺山柑果实中香草酸和原儿茶酸分别在0.001 50～0.009 00,0.005 80～0.034 8μg呈良好的线性关系,(r=0.999 9);平均加样回收率(n=3)分别为105%(RSD 1.90%)103%(RSD=1.81%)。结论:该方法操作简单、结果可靠、重复性好、定量准确,可用于刺山柑果实中原儿茶酸和香草酸的含量测定。

Objective： To establish a HPLC method for simultaneous determination of vanillic acid and protocatechuic acid in Capparis spinosa. Method： The samples were separated on a C18 column （4.6 mm×150 mm, 5 μm） , the mobile phase was consisted of methanol-0. 1% phosphoric acid with gradient elution at the flow rate of 0.8 mL ·min^ -1 and the detection wavelength was set at 258 nm. Result： The calibration curves for vanillic acid and protocatechuic acid were linear in the range of 0. 001 50-0. 009 00, 0. 005 80-0. 034 8 μg respectively. All the correlation coefficient were 0. 999 9, and the average recoveries （n = 3） were 105% and 103% with corresponding RSDs of 1.90% and 1.81% respectively. Conclusion： The method is simple, accurate and sensitive and can be used to determine the content of the vanillic acid and protocatechuic acid in C. spinosa.