HPLC测定明目颗粒中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1的含量

Content Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Ginsenoside Rb_1 in Mingmu Granule by HPLC

目的:建立明目颗粒中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法:采用HPLC,DikmaDiamonsil(钻石)C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈(A)-水(B)进行梯度洗脱,流速1 mL·min-1,检测波长203nm,柱温25℃,进样量10μL。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的线性范围分别为0.352～2.112μg(r=0.999 3),0.914～5.484μg(r=0.999 2),0.910～5.460μg(r=0.999 1);平均加样回收率分别为99.4%,102.72%,101.89%,RSD分别为2.56%,2.16%,2.16%。结论:本试验建立的测定方法简便、重复性好、精密度高,可用于该制剂的质量控制。

Objective： To establish a method for determination of notoginsenoside R1 , ginsenoside Rg1 ,ginsenoside Rb1 in Mingmu granule. Method： HPLC was used, Dikma Diamonsil Cls column （4.6 mm x 150 mm, 5μm）, mobile phase of acetonitrile and water with gradient elution, flow rate was 1.0 mL · min^-1 and detection wavelength was at 203 nm, column temperature was 25 ℃ , injection volume 10 μL. Result： Linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb, were 0. 352-2. 112 （r =0. 999 3）, 0. 914- 5. 484 （ r = 0. 999 2 ） , 0. 910-5. 460 μg （ r = 0. 999 1 ） , respectively. Their average recoveries were 99.4% （RSD 2.56% ）, 102.72% （RSD 2. 16% ） and 101.89% （RSD 2.16% ）, respectively. Conclusion： This established determination method was simple, accurate and reproducible, thus it could be used as quality control of Mingmu granule.