齐墩果酸脂质体包封率的测定

Determination of Entrapment Efficiency of Oleanolic Acid Liposomes

目的:建立齐墩果酸脂质体的包封率测定方法。方法:采用薄膜分散法制备脂质体,葡聚糖凝胶柱色谱法分离脂质体和游离药物,以蒸馏水和0.5%SLS为洗脱介质分别洗脱脂质体和游离药物,采用高效液相色谱法测定脂质体中齐墩果酸的含量。结果:齐墩果酸在0.302～30.2 mg·L-1,线性关系良好(r=0.999 9),平均回收率为99.92%,RSD 1.59%;葡聚糖凝胶柱色谱法分离游离药物平均柱回收率为100.22%,RSD 1.23%,平均柱加样回收率为99.04%,RSD 0.99%;样品的平均包封率为83.4%。结论:齐墩果酸脂质体包封率测定方法准确可信,可用于测定齐墩果酸脂质体的包封率。

Objective： To establish a method to determine the entrapment efficiency of oleanolic acid liposomes. Method： Oleanolic acid liposomes were prepared by film dispersion method. Entrapment efficiency was determined after the separation of the unentrapped drug by glucan gel column chromatography. Distilled water was used to elute liposomes, 0.5% SLS was used to elute unentrapped drug. The content of oleanolic acid was deterMined by HPLC. Result： The linear, range of oleanolic acid was 0. 302-30.2 mg · L^- 1 （ r = 0. 999 9 ） . The average recovery was 99.92% （RSD 1.59% ）. The recovery of glucan gel column chromatography was 100.22% （RSD 1.23% ）, and the recovery of oleanolic acid was 99.04% （RSD 0.99% ）. The average entrapment efficiency of oleanolic acid liposomes was 83.4%. Conclusion ： This method is accurate, credible and it can be used to determine the entrapment efficiency of oleanolic acid liposomes.