HPLC测定梓木草饮片中尿囊素的含量

Determination of Allantoin in Lithospermun Zollingeri by HPLC

目的:建立梓木草饮片中尿囊素含量测定的方法。方法:采用HPLC测定。色谱柱Hypersil ODS-C18柱(4.6mm×250 mm,5μm),流动相甲醇-水(5∶95),流速0.2 mL·min-1,检测波长210 nm,柱温30℃,进样量20μL。结果:尿囊素在12.32～61.60μg呈良好的线性关系(r=0.999 9);尿囊素平均加样回收率为98.1%,RSD 1.55%(n=6)。结论:该方法可以作为梓木草饮片的质量控制,不同产地梓木草饮片中尿囊素含量差异较大。

Objective ： To establish a method for the determination of allantoin in Lithospermun zollingeri. Method： Separation and determination of allantoin were accessed by using an ODS-C18 column and a mobile phase of methanol-water at a ratio of 5 ： 95. The flow rate was 0.2 mL · min^-1, temperature at 30 ℃ and detection wavelength at 210 nm. Result： A good linearity of allantoin was achieved in the range of 12.32-61.60 μg （r = 0. 999 9） . The mean value of recovery was 98.1% , RSD was 1.55%. Conclusion： The method in this paper could provide an evidence for quality control of L. zoUingeri and there is comparatively large difference of content of allantoin in L. zoUingeri from different producing areas.