摘要
目的:建立驱白白热斯酊中补骨脂素和异补骨脂素的含量测定方法,以控制该产品的质量。方法:采用HPLC法,Scienhome kromasil ODS-1色谱柱(4.6 mm×250 mm,5μm),柱温40℃,流动相甲醇-水(55∶45),流速1.0 mL·min-1,检测波长246 nm。结果:补骨脂素和异补骨脂素分别在6.12~18.36 mg·L-1,在6~18 mg·L-1线性关系良好,相关系数分别为r补骨脂素=0.999 0,r异补骨脂素=0.999 1。两组份的回收率分别为补骨脂素101.57%,RSD 1.6%;异补骨脂素100.39%,RSD1.5%。结论:该法可同时测定补骨脂素和异补骨脂素,方法简便易行、准确可靠,可用于驱白白热斯酊的质量控制。
Objective: To establish a reversed-phase HPLC method for the determination of psoralen and isopsoralen in Qubaibairesi tincture. Method: The method of RP-HPLC was carried out with Scienhome kromasil ODS-1 column (4.6 mm ×250 mm, 5 μm) ; the flow rate of the mobile phase of methanol-water (55: 45) was 1.0 mL·min^-1; the column temperature was maintained at 40 ℃ and the detection wavelength was set at 246 nm. Result: The linear ranges of psoralen and isopsoralen were 6. 12-18.36 mg ·L-1 (r =0. 999 0) and 6-18 mg ·L^-1 (r =0. 999 1) respectively. The recoveries were 101.57% (psoralen) were 1.6% (psoralen) and 1.5% (isopsoralen). Conclusion: to control quality of Qubaibairesi tincture. and 100.39% (isopsoralen) The method is simple, accurate , and their RSDs and can be used
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期114-116,共3页
Chinese Journal of Experimental Traditional Medical Formulae