HPLC测定不同产地牛蒡草中绿原酸含量

Determination of Chlorogenic Acid in Arctium lappa by HPLC

目的:建立测定牛蒡草主要活性成分绿原酸含量的方法,并比较不同产地牛蒡草绿原酸的含量差异,为牛蒡草质量控制提供依据。方法:采用Agilent C18色谱柱(4.6 mm×150 mm,5μm),以乙腈-0.4%磷酸水溶液(8∶92)为流动相,检测波长327 nm,体积流量1.0 mL·min-1,柱温30℃,进样量10μL,外标法定量。结果:绿原酸得到良好分离,在0.041 8～4.18μg与峰面积积分值呈良好的线性关系,平均加样回收率为100.3%。结论:该方法简便、准确、分离度良好,为牛蒡草的质量控制提供了依据。

Objective： To establish an HPLC method to determine the content of chlorogenic acid in Arctium lappa, and to compare the content in A. lappa which from different regions. Method： HPLC was performed on an Agilent ClS analytical column （4.6 mm × 150 mm, 5 μm） at 30℃ with acetonitrile-0.4% phosphoric acid solution as the mobile phase; the detection wavelength was 372 nm and the flow rate was 1.0 mL ·min^-1. Result： Chlorogenic acid was separated perfectly, and its linearity was good in ranges of 0. 041 8-4. 18 μg. The average recovery was 100.3%. Conclusion： The validated method is simple, accurate, and had a good resoulution. It can be able to provide the basis for the ＇quality control of A. lappa.