摘要
目的:研究滋阴地黄丸对兔脉络膜组织中血管内皮生长因子(VEGF),整合素(aVβ3),组织因子(TF)表达的影响。方法:用激光光凝建立脉络膜新生血管(CNV)动物模型,将动物分为4组,分别为空白对照组、模型对照组、滋阴地黄丸高、低剂量组(39,9.75 g·kg-1),连续ig 30 d后取右眼球进行免疫组织化学染色,测定脉络膜中VEGF,aVβ3,TF的表达。结果:模型对照组兔眼脉络膜组织中VEGF,aVβ3,TF均呈强阳性表达,滋阴地黄丸低、高剂量组中VEGF,aVβ3,TF则呈弱阳性或阴性表达。与模型组比较,滋阴地黄丸低剂量组明显抑制兔脉络膜中VEFG的表达(P<0.05),显著地抑制了aVβ3和TF的表达(P<0.01),滋阴地黄丸高剂量组也显著地抑制了VEGF,aVβ3和TF的表达(P<0.01)。结论:滋阴地黄丸通过抑制脉络膜中VEGF,aVβ3,TF的表达而抑制了CNV的形成。
Objective： To study the effects of Ziyin Dihaung Wan on the expression of vascular endothelial growth factor （VEGF）, integrin （aVβ3） and tissue factor （TF） in rabbit choroids tissue. Method： Chorodial neovascularization （CNV） model was induced by laser, and then administrated with 9.75, 39 g .kg^-1Ziyin Dihaung Wan for 30 days and then rabbit eyeballs were performed with immunohistochemical stainingfor determination of the expression of VEGF, aVβ3 and TF in choroid. Result： Compared with the strongly positive expression of VEGF, aVβ3 and TF in model group rabbit choroidal tissue, the expression of VEGF and aVβ3 and TF was weakly positive in low, high dose Ziyin Dihaung Wan treatment groups. Ziyin Dihuang Wan at 9.75 g ·kg^-1 dose inhibited the expression of VEGF, aVβ3 and TF significantly （P 〈 0.05）. Ziyin Dihuangwan at 39 g ·kg^-1 dose inhibited the expression of VEGF, aVβ3 and TF significantly （P 〈0, 01 ）. Conclusion： Ziyin Dihuang Wan inhibited CNV by inhibiting the expression of VEGF, aVβ3 and TF in rabbit choroids tissue.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期160-163,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
孟河医派方药传承及开发研究(BM2008152-KF04)
