半夏泻心汤及其拆方对胃腺癌SGC-7901细胞周期和凋亡的影响

Effects of Banxia Xiexin Decoction and its Components on Cell Cycle and Apoptosis of Gastric Cancer SGC-7901 Cells

目的:从细胞增殖、凋亡、细胞周期角度,研究半夏泻心汤治疗胃癌机制。方法:把半夏泻心汤拆分为苦降、辛开和补益3个组方,1×104细胞/孔接种于96孔板,药物浓度为4,2,0.5,0.125 g·L-1,MTT法检测细胞增殖;1×105/孔接种于6孔板,全方和苦降高、低浓度为0.5,0.125 g·L-1,辛开和补益高、低浓度为4,2 g·L-1,流式细胞仪检测细胞周期和细胞凋亡。观察半夏泻心汤及其拆方对胃癌细胞SGC-7901增殖、细胞周期和凋亡的影响,并与5-氟尿嘧啶(5-Fluorouracil,5-Fu)比较。结果:半夏泻心汤全方及苦降、辛开组方能够抑制SGC-7901细胞增殖,全方IC50为1.645 g·L-1,苦降组为1.446 g·L-1,辛开组为3.867 g·L-1;它们增加SGC-7901细胞S期和G0-G1期百分比,促进细胞凋亡,高浓度强于低浓度,但补益组方对此没有作用。结论:半夏泻心汤及其拆方能够一定程度的抑制细胞增殖,以苦降组为最,补益组作用甚微,其机制可能与药物阻滞细胞至G1/S期和诱导细胞凋亡有关。

Objective： Study the effects and mechanism of Banxia Xiexin decoction on gastric cancer. Method： Banxia Xiexin decoction was divided into Pungent openning （dried ginger, pinellia ternate） group, Bitter Descending （coptis chinensis, scutellaria） group and Invigoration （glycyrrhiza, ginseng） group by its different nature and flavor. 1 ×10^4cells/wells were planted at 96 wells plate, drug concentrations were 4, 2, 0.5, 0. 125 g·L^-1, cell proliferation was detected by MTT method. 1 × 10^5 cells/wells were planted in 6 wells plate. The high and low concentration of whole Party and Bitter Descending drugs was respectively 0. 5, 0. 125 g · L^-1. The high and low concentration of Pungent openning and invigoration was respectively 4, 2 g ·L^-1. Effect of Banxia Xiexin decoction and compositions on gastric cancer SGC-7901 ceils proliferation, cell cycle and apoptosis ratio were detected and compared with known positive drug 5-fluorouracil （5-FU）. Result： Banxia Xiexin decoction group, Bitter Descending group and Pungent opening group could inhibit the proliferation of SGC-7901 cell, the IC50 values of its were 1. 645, 1. 446, 3. 867 g ·L^-1 respectively, and increased SGC-7901 cells in S phase and G0-G1 phase percentage with promoting apoptosis in dose-dependent manner, but invigoration group had no such effect. Conclusion ： Banxia Xiexin decoction and its compositions can inhibit the proliferation of SGC-7901 cell, Bitter Descending is strongest and Invigoration group is the weakest, which may be related to blocking of cells to G1/S phase and inducing cell apoptosis