新疆金鸡菊提取物对2型糖尿病小鼠氨基酸代谢谱的影响

Metabonomics Study on the Serum Amino Acid in Type 2 Diabetes Mice Treated by Extract of Coreopsis tinctoria

目的:研究新疆金鸡菊(Xinjiang Coreopsis tinctoria)乙酸乙酯提取物对2型糖尿病小鼠血清氨基酸代谢谱的影响。方法:高脂高糖喂养联合腹腔注射链脲佐菌素(streptozotocin,STZ)建立2型糖尿病模型,造模成功后随机分组,灌胃给予新疆金鸡菊提取物8 g·kg-1·d-1,阳性药二甲双胍0.16 g·kg-1·d-1,每周测空腹血糖(FBG),给药4周后处死,收集小鼠血清,检测甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平。采用柱前衍生高效液相色谱法(HPLC)检测小鼠血清中氨基酸代谢谱的变化。结果:结合SIMCA-P软件的偏最小二乘法判别(PLS-DA)对氨基酸数据进行分析,结果显示新疆金鸡菊乙酸乙酯提物给药后对血清氨基酸代谢谱的改变较模型组有很大区别,有4种氨基酸对组间贡献较大(牛磺酸、天冬氨酸、苏氨酸、蛋氨酸)。小鼠成模后体内氨基酸发生了明显变化。与空白组、模型组、阳性组相比,新疆金鸡菊乙酸乙酯给药组小鼠血清氨基酸代谢谱发生了显著变化。结论:新疆金鸡菊乙酸乙酯提取物显著影响小鼠血清中与糖尿病相关的氨基酸代谢标志物,为进一步阐释新疆金鸡菊抗糖尿病药理作用的研究提供了依据。

Objective： To investigate the serum amino acid variation in diabetic mice. Method： SPF mice were fed with high-fat diet for 4 weeks and then injected with streptozotocin （ STZ）. Mice were successively administrated with ethyl acetate extract Coreopsis tinctoria （ 8 g ·kg^-1·d^-1 ） for 30 days. HPLC was used for the analysis of amino acid metabolites in serum samples. These data were analyzed by partial least square-discrimiant analysis （ PLS-DA）. Result： Compared with the control mice, the metabolism profiles of amino acid in the model mice were significantly different from that of the control mice. Pattern recognition method was applied and 4 amino acid markers were different between all the groups. Conclusion： The amino acid metabolism profiling may reflect to certain extent the pathological changes of type 2 diabetic mice. This work can provide basis for further researches on the interpretation of drug property of evidence.