耐力胶囊对^(60)Co γ射线损伤小鼠的保护作用

Protective Effect of Naili Capsule on Irradiated Injury Induced by ^(60)Co γ-ray in Mice

目的:研究耐力胶囊对辐射损伤小鼠的保护作用,为开发有效的抗辐射药物提供实验依据。方法:雄性昆明种小鼠,分为辐射模型组、阳性药芪胶升白胶囊组(0.98 g·kg-1)及耐力胶囊低、中、高3个剂量(分别为1.82,3.64,7.28 g·kg-1)组,连续ig 7 d后,实验分两部分:经8.5 Gy60Coγ射线照射,观察耐力胶囊对小鼠30 d存活率,平均生存时间的影响;6.5 Gy60Coγ射线照射,观察耐力胶囊对辐射损伤小鼠的外周血细胞、骨髓有核细胞计数、免疫器官指数、骨髓组织病理的影响。结果:耐力胶囊中剂量组可以提高受照小鼠30 d存活率(提高存活率为37.5%),延长受照小鼠平均存活时间(24.88 d),保护系数达1.30;可以升高受照小鼠的外周血白细胞(辐射后3,7 d,WBC计数分别为1.34×109/L,0.89×109/L;),增加辐射后7 d小鼠免疫器官指数(胸腺和脾脏指数分别为0.68,0.98 mg·g-1)和骨髓有核细胞计数(5.89×106/股骨),减轻小鼠骨髓造血细胞的损伤。结论:耐力胶囊对60Coγ射线损伤小鼠具有保护作用。

Objective： To study the protective effect of Naili capsule on ^60Coγ-ray induced injury and proyide an experimental basis for the development of Naili capsule. Method： Male KM mice were divided into model group, the positive drug group （ dose 0.98 g ·kg^-1 ） and Naili capsule low, medium and high doses （for 1.82, 3.64, 7.28 g ·kg^-1） group, ig, for 7 days consecutively. Experiment was divided into two parts： mice were radiated by 8.5 Gy of ^60Coγ ray radiation, survival rate of 30 days was observed and the average survival time of mice was calculated ; mice were radiated by 6.5Gy ^60Coγ ray radiation. Peripheral blood cells, bone marrow nucleated cell count, the role of the index of immune organs, bone marrow histopathology of mice were detected on 7 days after radiation. Result： The 30-day survival rate （improved survival was 37.5% ） and the mean survival time （24.88 d） of mice administered Naili capsule （for 3.64 g·kg^-1） were all much higher than those of the model group and protection factor was 1.30. White blood cells of mice irradiated were improved （ 1.34 × 10^9/L on 3 days after radiation and 0. 89 × 10^9/L on 7 days after radiation） and the marrow nucleated cell counts could be increased （5.89 × 10^6 in every femur）. The damage of bone marrow hematopoietie cells in mice could be reduced.Conclusion： Naili capsule has significant radioprotective effect in mice.