痛风舒宁片治疗高尿酸血症的实验研究

Experimental Study of Tongfeng Shuning Tablet for Treatment of Hyperuricemia

目的:探讨痛风舒宁片治疗高尿酸血症的药理作用。方法:痛风舒宁片低、中、高剂量(1.1,2.2,4.4 g·kg-1)和别嘌醇片(40 mg·kg-1)ig连续7 d后,建立尿酸致小鼠高尿酸血症模型,观测对血清尿酸的作用;痛风舒宁片低、中、高剂量(0.75,1.50,3.00 g·kg-1)和别嘌醇片(20 mg·kg-1)ig连续7 d,同时建立腺嘌呤+乙胺丁醇致大白鼠高尿酸血症模型,观察对血清尿酸(UA)、尿素氮(BUN)、肌酐(Cre)的作用,测定肾脏指数并进行肾脏病理组织学检查。结果:痛风舒宁片中、高剂量组小鼠血清UA分别为[(124.00±57.33),(56.27±42.24)μmol.L-1],明显低于模型组[(310.50±74.88)μmol.L-1](P<0.05,P<0.01)。痛风舒宁片高剂量组大鼠血清UA为(86.10±32.09)μmol.L-1,明显低于模型组(137.70±43.21)μmol.L-1(P<0.05);痛风舒宁片中、高剂量组Cre水平为[(45.80±8.46),(48.50±6.80)μmol.L-1],明显低于模型组(59.60±13.53)μmol.L-1(P<0.05,P<0.05);痛风舒宁片低、中、高剂量能改善肾实质病变程度(P<0.05,P<0.01,P<0.01)以及降低肾脏指数(P<0.05,P<0.05,P<0.05)。结论:痛风舒宁片有显著的抗高尿酸血症的作用。

Objective： To observe the effect of Tongfeng Shuning tablet （TST） on hyperuricemia in mice. Method： TST （1.1, 2.2, 4.4 g ·kg^-1） and allopurinol tablet （40 mg·kg^-1） were given ig. for 7 d in mice. Hyperuricemia model in mice was established by uric acid （UA） , and UA in serum was determined. TST （0.75, 1.50, 3.00 g ·kg^-1） and allopurinol tablet （20 mg ·kg^-1） were given ig. for 7 d in rats, meanwhile, hyperuricemia in rats was established by adenine and ethambutol. UA, blood urea nitrogen （BUN） and creatinine （Cre） in serum were determined, kidney weight index and histopathological changes were also observed. Result： Compared with the mice model UA [ （310.50 ±74.88） μmol ·L^-1] , UA levels in middle and high doses of TST were [ （124.00±57.33）, （56.27 ±42.24） μmol ·L^-1] （P〈0.05, P〈0.01）. The UA level in high dose of TST was 86. 10 ± 32.09 μmol ·L^-1 lower than that （ 137.70 ± 43.21μmol ·L^-1 ） of the rat model （P 〈 0.05）. The level of Cre in middle and high dose of TST were [ （45.80 ±8.46） , （48.50 ±6.80）μmol ·L^-1] , lower than that [ （59.60 ± 13.53） μmol ·L^-1] in the rat model （P 〈0.05, P 〈0.05）. All doses of TST could decrease the kidney weight index （P 〈 0.05, P 〈 0.05, P 〈 0. 05 ） and relieve kidney histopathological damage （P 〈 0.05, P 〈0. 01, P 〈0.01）. Conclusion： TST had significant effect on hyperuricemia.