芸芥自交亲和性的遗传及自交亲和基因mRNA差异显示分析

Analysis of self-compatibility genes by mRNA differential display technique and inheritance of self-compatibility in E.sativa Mill

以8份芸芥自交亲和系与4份芸芥自交不亲和系为试验材料,通过正交和反交方式共组配了18个不同的杂交组合,调查了F1群体的自交亲和性与否,及1 424株F2群体和626株BC1群体的性状分离情况。同时,还采用mRNA差异显示技术研究了芸芥自交亲和基因的表达器官,分离、筛选了与自交亲和性相关的特异基因片段。结果表明:芸芥自交亲和性与细胞质遗传无关,而是由一对核基因控制的简单隐性遗传性状;芸芥自交亲和基因的表达不属于组成性表达,而属于组织特异性表达;在芸芥自交亲和系的柱头cDNA扩增中共得到3条差异cDNA片段,其与芸芥自交亲和基因有密切关系。

18 hybridized combinations were made between 8 self-compatible lines and 4 self-incompatible lines, and inheritance of self-compatibility in E. sativa Mill was analysed. Furthermore, expresstion tissue of self-compatible genes was preliminary analysed, and specific eDNA fragments related to self-compatibility were isolated and screened by using mRNA differential display technique. The results showed that all the F1 plants were self-incompatible, and the F2 and BC1 population segregated in ratio. Through chi square test, the segregation ratios of self-incompatibility to self-compatibility in F2 and BC1 population were coincided with the ratio of 1：3 and 1 ： 1, respectively. Therefore, it was inferred that the hereditary of self-compatibility in Yunjie was controlled by one pair recessive nucleus gene. Self-compatible genes of Yunjie was not constitutive expression, but that belonged to specific tissue expression. Self-compatible lines had 3 differentially expressed bands which exhibited stably with different primers, these eDNA bands were closely related to self- compatibility of Yunjie.