HPLC法测定紫果西番莲叶中异牡荆素的含量

Detection of isovitexin content in leaves of passiflora edulisby high performance liquid chromatography

目的应用HPLC法,对紫果西番莲叶中异牡荆素的含量进行测定。方法首先采用硅胶柱色谱进行分离纯化;采用HPLC,Hypersil ODS C18色谱柱(4.6×250 mm,5μm);乙腈-0.1磷酸(18∶82)为流动相;流速1.0 ml/min;进样量:10μl,检测波长350 nm。结果异牡荆素在0.012～0.120 mg/ml范围内时,呈现良好线性关系(R2=0.997);平均回收率为98.26%,RSD 1.4%;紫果西番莲叶中异牡荆素的平均含量为0.0873%。结论该方法准确、可靠、重复性良好,可用于西番莲属植物中异牡荆素含量的测定。

Objective To detect the content of isovitexin in the leaves of Passiflora edulis by high performance liquid chromatography （HPLC）. Methods Firstly, the extraction and purification were carried out by silica gel column chromatography. HPLC separation was carried out in Hypersil ODS C ls column （4.6 × 250 mm, 5 μm） with the mobile phase of acetonitrile - 0.1% H3PO4 solution （18： 82）, and the flow rate was 1.0 ml/min. The sample size was 10 μl, and the detection wavelength was 350 nm. Results Within the range of 0. 012 - 0. 120 mg/ml, isovitexin had a good linear relationship （R2 = 0. 997 ）. The average recovery was 98.26%, and RSD was 1.4%. The average content of isovitexin was 0.0873%. Conclusion The method is accurate, reliable, and repeatable, and can be used to detect the content of isovitexin in the leaves of passiflora edulis.