氮、磷对富油荒漠微藻混养生长及总脂的影响

Effects of Nitrogen and Phosphorus on the Mixotrophic Growth and Total Lipid of High-lipid Desert Microalgae

从新疆荒漠化土样中筛选出1株富油荒漠蛋白核小球藻(Chlorella pyrenoidosa),在自养条件下其生物量产率和油脂产率分别为29.0mg/(L.d)和4.6mg/(L.d)。实验考察了自养、异养、混养3种培养方式对荒漠蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累的影响,确定混养为其最佳培养方式。在此基础上探讨了氮、磷对荒漠蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累的影响,实验结果表明:荒漠蛋白核小球藻(Chlo-rella pyrenoidosa)混养的最优氮浓度为200mg/L,磷浓度为4mg/L;在最适氮磷比为136.7条件下,荒漠蛋白核小球藻(Chlorella pyrenoidosa)的最大生物量产率和油脂产率分别可达154.2mg/(L.d)和46.3mg/(L.d),为对照组的5.3倍和10.1倍。

A high-lipid desert microalgae Chlorella pyrenoidosa was isolated from the soil samples of desertification regions in Xinjiang.Its biomass and lipid productivity were 29.0 mg/（L·d） and 4.6 mg/（L·d） under autotrophic condition,respectively.The effects of different cultivation methods on cell growth as well as lipid accumulation of C.pyrenoidosa were investigated.The results showed that the optimal method was mixotrophic cultivation.The N and P in medium were further investigated,and the optimal nitrogen and phosphorus were determined as NaNO3（nitrogen concentration of 200 mg/L） and K2HPO4（phosphorus concentration of 4 mg/L）,respectively.Under this optimum conditions,the highest biomass and lipid productivity reached to 154.2 mg/（L·d） and 46.3 mg/（L·d）,respectively,which were 5.3 and 10.1times as much as the control.