双层膜放大的电流型癌胚抗原免疫传感器的研究

Double Membrane Amplification of the Amperometric Immunosensor for Sensing Carcinoembryonic Antigen

以金电极为电极材料,纳米铂/普鲁士蓝复合物膜为固载基底,癌胚抗体(anti-CEA)为模板免疫蛋白质分子,研制成一种高灵敏电流型癌胚抗原免疫传感器。该传感器以辣根过氧化氢酶(HRP)代替牛血清白蛋白封闭非特异性结合位点,放大了电流信号,提高了电极的灵敏度,并采用循环伏安技术对电极的制备过程进行了研究。在优化的实验条件下,该传感器响应的峰电流值与癌胚抗原浓度在1.0～80.0ng/mL的范围内保持良好的线性关系,检测限为0.4ng/mL。

A new amperometric immunosensor for the determination of carcinoembryonic antigen（CEA） was developed by means of immobilizing carcinoembryonic antibody onto the nano-Pt/ Prussian-blue-modified composite film.The research substituted Horseradish peroxidase（HRP） for bovine serum albumin（BSA） to block nonspecific binding sites and amplify the current signal of the antigen-antibody reaction.Cyclic voltammetry（CV） was employed to characterize the assembly process.Under the optimized conditions,the response current of the proposed immunosensor has a good linear relationship with CEA in the concentration ranges from 1.0 to 80.0 ng/mL with a detection limit of 0.2 ng/mL.