羊栖菜岩藻黄质色素的抗氧化性研究

Study on antioxidant activities of fucoxanthin pigments from Sargassum Fusiform

为研究羊栖菜超临界CO2提取的岩藻黄质色素粗品的抗氧化活性,首先应用分光光度法测定了岩藻黄质粗品的还原能力、对DPPH·清除率以及抗脂质过氧化功能,再通过化学发光法测定了岩藻黄质粗品清除·OH、O-2·和H2O2的性能。结果表明,羊栖菜岩藻黄质色素粗品浓度与抗氧化活性均呈良好的量效关系,对·OH、O-2·、DPPH·与H2O2具有较强的清除能力,还原能力表现较弱。其中岩藻黄质粗品·OH的IC50为386.29μg/mL,清除率强于对照品VC,弱于VE;DPPH·的IC50为565.2μg/mL,约为对照品VE的211.4%;O-2·的IC50为615.71μg/mL,H2O2的IC50为658.74μg/mL,清除率均小于VE、VC对照品。岩藻黄质粗品抗脂质过氧化功效为BHT的50%左右,远强于VC。因此,羊栖菜岩藻黄质色素粗品具有良好的抗氧化活性,有望成为天然抗氧化剂得到开发利用。

In order to study the antioxidant activities of fucoxanthin products extracted by supercritical carbon dioxide from Sargassum Fusiform, first, the experiments of reducing power, the clean- up rates on DPPH ~ and preventing effect on lipid peroxidation were performed using spectrophotometry and then the function of clean-up on · OH,O2^- free radicals and H2O; were studied by the method of chemiluminescence.Results indicated that fucoxanthin products of Sargassum Fusiform had doseresponse relationship with antioxidant activities,throwing a weak reducing power but strong clean-up rates on·OH, O2^- - free radicals, H202 and DPPH · The IC50 for clean -up rates on · OH was 386.29μg/mL, superior to Vc ＇ s but below VE＇ s, the IC50 to DPPH was 565.2μg/mL, about 211.4% of the VE＇ S and the IC5o to O2^- ·was 615.7] μg/mL, less than that of VE and Vc.Preventing effect on lipid peroxidation was about 1/2 times of BHT and much more powerful than Vc.Therefore, fucoxanthin products from Sargassum Fusiform had well antioxidant activities, and could be developed and used as native antioxidant hopefully.