摘要
为研究羊栖菜超临界CO2提取的岩藻黄质色素粗品的抗氧化活性,首先应用分光光度法测定了岩藻黄质粗品的还原能力、对DPPH·清除率以及抗脂质过氧化功能,再通过化学发光法测定了岩藻黄质粗品清除·OH、O-2·和H2O2的性能。结果表明,羊栖菜岩藻黄质色素粗品浓度与抗氧化活性均呈良好的量效关系,对·OH、O-2·、DPPH·与H2O2具有较强的清除能力,还原能力表现较弱。其中岩藻黄质粗品·OH的IC50为386.29μg/mL,清除率强于对照品VC,弱于VE;DPPH·的IC50为565.2μg/mL,约为对照品VE的211.4%;O-2·的IC50为615.71μg/mL,H2O2的IC50为658.74μg/mL,清除率均小于VE、VC对照品。岩藻黄质粗品抗脂质过氧化功效为BHT的50%左右,远强于VC。因此,羊栖菜岩藻黄质色素粗品具有良好的抗氧化活性,有望成为天然抗氧化剂得到开发利用。
In order to study the antioxidant activities of fucoxanthin products extracted by supercritical carbon dioxide from Sargassum Fusiform, first, the experiments of reducing power, the clean- up rates on DPPH ~ and preventing effect on lipid peroxidation were performed using spectrophotometry and then the function of clean-up on · OH,O2^- free radicals and H2O; were studied by the method of chemiluminescence.Results indicated that fucoxanthin products of Sargassum Fusiform had doseresponse relationship with antioxidant activities,throwing a weak reducing power but strong clean-up rates on·OH, O2^- - free radicals, H202 and DPPH · The IC50 for clean -up rates on · OH was 386.29μg/mL, superior to Vc ' s but below VE' s, the IC50 to DPPH was 565.2μg/mL, about 211.4% of the VE' S and the IC5o to O2^- ·was 615.7] μg/mL, less than that of VE and Vc.Preventing effect on lipid peroxidation was about 1/2 times of BHT and much more powerful than Vc.Therefore, fucoxanthin products from Sargassum Fusiform had well antioxidant activities, and could be developed and used as native antioxidant hopefully.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第23期125-128,共4页
Science and Technology of Food Industry
基金
浙江省水产品加工技术研究联合重点实验室资助(2011E10002-05)
关键词
羊栖菜
岩藻黄质
抗氧化性
Sargassum Fusiform
fucoxanthin
antioxidant activities