响应面法优化南极磷虾壳中虾青素提取条件的研究

Optimization of extracting astaxanthin from Antarctic krill shell by response surface methodology

以南极磷虾壳为原料,采用响应面法研究虾青素的最优提取条件,为南极磷虾的加工利用提供依据。以虾青素提取率为指标,在单因素实验基础上确定实验因素及其水平,并设计三因素三水平响应面实验,研究料液比、提取温度和提取时间对提取率的影响。结果显示,最佳工艺条件是料液比为1∶5(g/mL)、提取温度为30℃、提取时间为7h,提取级数为2级,此实验范围内虾青素的提取率达到最大值131.56μg/g。

In order to provide the basis for the deep processing of Antarctic krill, response surface methodology （RSM）was used to optimize extraction parameters to astaxanthin from Antarctic krill shell.Based on the singlefactor experiment,three variables,such as ratio of krill shell wet weight to dichloromethane,extraction temperature and extraction time,were optimized.The results showed that the ratio of kritl shell wet weight to dichloromethane at ]：Sg/mL, shook at the constant temperature（30℃）for 7h, and extracted for 2 times,the extraction volume of astaxanthin achieve the maximum （ 131.56μg/g ）.