薯蔓多糖的纯化工艺

Purification technology of polysaccharide from sweet potato vines

采用活性炭脱色,选取时间、温度、活性炭用量3个因素进行正交实验,用脱色率和多糖含量作为考察指标。采用三氯乙酸法和Sevage法去除蛋白质,主要研究其浓度与体积比对蛋白质去除率和多糖损失率的影响。结果表明:40℃、40min、活性炭用量为1.0%时去除色素效果最好,脱色率为21.98%,多糖保留率为94.09%。薯蔓多糖溶液与5%TCA溶液体积比为1∶2时,去除蛋白质效果较好;Sevage(氯仿∶正丁醇=4∶1)溶液与薯蔓多糖溶液体积比为1∶1时,去除蛋白质效果较好。

Polysaccharide from sweet potato vine was decolored by active carbon and the orthogonal test was used with three variables：time,temperature and amount of active carbon.The rate of decolorization and polysaccharide content were chosen as the decolorization criteria.The protein was removed using trichloroacetic acid method and Sevage method.This research emphasized on its effect on the loss rate of removed protein and polysaccharide by changing solutions concentration and volume ratio.The results showed that the decolorizations maximum value was 21.98% and retention rate of polysaccharide was 94.09% with the temperature of 40℃,40min and L0% amount of active carbon.As the ratio of sweet potato vine polysaccharide solution to 5% TCA solution was 1 ：2, the effect of removing protein was better. As the ratio of sevage （ chloroform： nbutanol = 4 ： 1） solution to sweet potato vines polysaccharide solution was 1：1 ,the effect of removing protein was better.