单一pH法、pH示差法和差减法快速测定干红葡萄酒中总花色苷含量的比较

Comparison of single pH method,pH-differential method and substraction method for determining content of anthocyanins from red wine

为了寻求快速、经济地测定干红葡萄酒中总花色苷含量的方法,采用单一pH法、pH示差法和差减法对比分析3种方法测定数据的差异性。结果表明,3种方法测定的吸光度与浓度间均具有良好的线性关系,其相关系数R分别为0.9977、0.9988和0.9984,测定结果没有显著性差异(p<0.05)。3种方法均能用于干红葡萄酒总花色苷含量的快速测定。

In order to find a quick and economical method for determining total content of anthocyanins from red wine,single pH method,pH-differential method and substraction method were used and the difference between the results of the three methods was compared.The results indicated that linear relation between absorbanceand concentration determined by that three methods were matched well,their related coefficient are 0.9977,0.9988 and 0.9984,and there were no significant differences between them（ p 〈 0.05）.All of these methods could be used to determine total content of anthocyanins from red wine.