大鼠腹腔感染致早期肺损伤的实验研究

Experimental study of early lung injury caused by abdominal infection in rats

目的 探讨肠源性感染致早期肺损伤的作用机理。方法 采用大鼠盲肠结扎并穿孔 (CLP)造成腹腔感染。检测肺毛细血管通透性 ,取支气管肺泡灌洗液 (BALF)进行细胞学分析 ,检测血浆、肺组织和BALF的内毒素和肿瘤坏死因子 (TNF)。结果 肺毛细血管通透性和BALF的中性粒细胞百分率逐渐增加 ,时间越长越明显。血浆、肺组织和BALF的内毒素逐渐增加 ,三者之间两两显著相关 ;TNF也逐渐增加 ,肺组织和BALF的TNF显著相关 ,两者与血浆的TNF无明显相关性。结论 腹腔感染导致肺组织中内毒素增加 ,中性粒细胞积聚 ,TNF释放 ,肺产生炎性反应。

Objective To study the mechanism of early lung injury caused by intestinal infection in rats.Methods The cecal ligation and perforation(CLP) was utilized to make the abdominal infection in rats.The pulmonary vascular permeability and the differential cell count in bronchoalveolar lavage fluid(BALF) were examined. The concentrations of endotoxin and tumor necrosis factor(TNF) in plasma, lung and BALF were detected.Results The pulmonary vascular permeability and the neutrophil percentage of BALF increased progressively. The concentrations of endotoxin and TNF in plasma, lung and BALF significantly increased. The significant correlation was respectively present between the endotoxic levels of plasma, lung and BALF and between the TNF levels of lung and BALF, but not between the TNF level of plasma and one of lung or BALF.Conclusion The abdominal infection might lead to increase of endotoxin, accummulation of neutrophils and release of TNF in lung, which were contributed to the inflammatory reaction of lung. [