肢体缺血再灌注致肠损伤及甘露醇的保护作用

Protective effect of mannitol on intestinal injury after lower limb ischemic reperfusion

目的 通过动物实验观察甘露醇对肢体缺血再灌注所致肠损伤的保护作用。方法 80只Wistar大白鼠随机分成正常对照组 ,缺血 3小时和缺血 3小时用甘露醇 3个组。实验组于缺血、再灌注 1、2、3和 2 4小时取材光镜对比观察和组织学评估。结果 肢体缺血和再灌注后 ,实验组肠粘膜固有层毛细血管扩张、充血、大量多核白细胞浸润。大肠和小肠粘膜厚度和隐窝深度明显减少 (P <0 0 1) ,小肠绒毛高度相对增加 (P <0 0 1) ,隐窝细胞有变性坏死 ,用甘露醇组的病变明显轻于未用甘露醇组。结论 甘露醇能有效地减轻肢体缺血再灌注所致的肠损伤。

Objective An experimental study to observe protective effect of Mannitol on intestinal injury after lower limb ischemic reperfusion injury.Methods Eighty rats were divided into control group,ischemia 3 hours and ischemia 3 hours group treated with mannitol.The histological assessment of the bowel were undertaken at 0,1,2,3 and 24 hours after reperfusion.Result It was found that microvascular congestion aggregation of large amount of polymorphonuclear neutrophil(PMN) within the lamina propria was observed in experimental groups at the different times.The bowel showed a significant decreased in mucosal thickness and crypt depth,and a significant increased in villus height of small bowel compared with control.Degeneration and necrosis of crypt cells were found in experimental groups.There was a significant lessening of the injury in the group treated with Mannitol.Conclusion These data suggest that Mannitol can alleviat the intestinal injury after lower limb ischemic reperfusion. [