CYP2D6基因克隆及在自身免疫性肝炎中的应用

CLONING OF CYP2D6 AND ITS APPLICATION IN AUTOIMMUNE HEPATITIS

采用基因克隆与表达技术制备重组人 CYP2 D6抗原 ,建立免疫印迹法 ,检测几组肝炎患者中的抗CYP2 D6抗体。 6 0 5例 AL T升高的成人肝炎患者组内 CYP2 D6自身抗体阳性检出率为 3.97% (2 4/ 6 0 5 ) ,5 0例AL T升高的小儿肝炎患者组内阳性检出率为 1 2 % (6 / 5 0 )。两组比较 ,经统计学处理差异有显著性意义 (P<0 .0 1 )。5 0名正常人及 40例 HCV RNA阳性的肝炎患者中无一例发现该自身抗体阳性。检出的 30例 CYP2 D6自身抗体阳性患者 ,30 %合并有 HBV感染。结果提示 :CYP2

Using gene cloning and expression technique, we established Immunblot to detect anti CYP2D6 antibody in the sera of patients with hepatitis. CYP2D6 autoantibody was found in 3.9%(24/605) of adults with heptitis, and 12.00%(6/50) of children hepatitis. The positive percentage of CYP2D6 autoantibody was significantly different ( P <0.01) between the two groups. On the contrary, neither 40 patients with HCV infection, nor 50 healthy controls were positive for CYP2D6 autoantibody. 30% of patients (9/30) with CYP2D6 autoantibody were positive for hepatitis B virus (HBV) in their sera detected by PCR, which suggested there was some relationship between HBV infection and autoimmune hepatitis.