Glob-N对胶质瘤细胞株SWO-38生长的体外实验研究

In vitro study of Glob-N on the growth of glioma cell line SWO-38

目的 :为了探讨正常及肿瘤相关中性鞘糖脂 (N GSLs)对肿瘤细胞生长的调控作用。方法 :采用改良的Hakomori方法提取、纯化正常人脑、牛脑、正常人“O”型血红细胞膜的N GSLs ,运用柱层析的方法分离正常人脑、牛脑、红细胞膜的红细胞糖苷脂 (Glob N)及人脑二己糖神经鞘氨醇 (CDH) ,MTT法检测各种来源的Glob N及人脑CDH对胶质瘤细胞株SWO 38生长的影响。结果 :三种不同来源的Glob N均能抑制SWO 38细胞的增殖。其中以正常人脑的作用最强 ,红细胞来源的作用次之 ,牛脑来源的最弱 ,而人脑CDH则缺乏抑制作用。结论 :Glob N是一种胶质瘤细胞的生长抑制因子 。

Objective:To investigate the effect of GlobN on thegrowth of glioma cell line SWO38 in vitroMethods:Neutral glycosphingolipids(NGSLs) were extracted and purified separately from normal human brain, membrane of human red blood cell type “O”and cow brain according to the modified Hakamori methodGlobN and CDH were further separated by using columnchromatography for gradient elution with methanol/chloroform solutionCytotoxicity effect of GlobN and CDH on SWO38 cells was determined by MTT assay invitroResults:All the GlobN of three different sources showed cytotoxicity to SWO38 cell line with IC 50 (24 hours incubation) of 1224, 1829 and4816 μg/ml, respectively, while CDH appeared no inhibitory effectConclusion:GlobN exhibites inhibitory effect on glioma cells in vitro, that may relate to its stucture