人实体瘤BUdR离体标记BUdR/DNA双参数FCM测定Tpot的初步研究

Preliminary studies of determining Tpot in human solid tumor by BUdR incorporation in vitro and BUdR/DNA bivariate FCM

目的 :探索人实体瘤标本BUdR离体标记BUdR/DNA双参数FCM测定Tpot的方法。方法 :选用头颈肿瘤活检标本及直肠癌手术标本行离体溴脱氧尿嘧啶核苷 (bromodeoxyuridine ,BUdR)标记双参数流式细胞术(flowcytometry ,FCM )测定肿瘤潜在倍增时间 (Potentialdoublingtime,Tpot)。 结果 :头颈肿瘤活检标本标记结果为阴性 ,直肠癌手术标本离体标记结果与文献报道的在体标记的结果有较大的差异。结论 :Tpot测定应尽可能采用在体BUdR标记 。

Objective:To explore the way of determining Tpot in the human solid tumor using the method of BUdR incorporation in vitro and BUdR/DNA bivariate FCMMethods:The bioptic samples of head and neck cancer and the surgical specimens of rectum cancer were used for Tpot measurement by BUdR incorporation in vitro and BUdR/DNA bivariate FCMResults:There was no successfullabeling in the bioptic samples of head and neck cancerThe labeling value insurgical specimens of rectum cancer was obviously different from the results ofthe in vivo labeling of other authors reportConclusions:Tpot measured by in vivo BUdR labeling should be developed, and the more appopriate method of in vitro labeling should be further investigated