一种新的胸腺素α原cDNA

Sequence of a new prothymosin α cDNA

目的 :我们克隆了有中国人特征的胸腺素 α原 (prothymosinα,PTMAα)全长 c DNA,并进行测序分析。方法 :从健康中国人 PBMC po1y(A) + RNA中采用 RT- PCR法克隆 hu PTMAα c DNA,酶切鉴定 ,序列分析。结果 :经测序后发现该基因是一个与国外的高加索人种胸腺素 α原有 86 .4%同源性的新基因。结论 :从中国人中新发现了一个 hu PTMAα基因 ,并于日前被美国 NCBI(National Center of Biotechnology Inform ation) Gen Bank收入 ,获得登录号 (Accession:AF170 2 94)

Objective: To clone a prothymosin α (PTMA α) which has important biological functions and analyze its sequence. Methods: A huPTMA α cDNA was cloned from poly(A) + RNA of healthy Chinese peripheral blood monocyte cells by RT PCR and the sequence was analyzed. Results: It was a new subtype of huPTMA α and has 86.4% homology with Genbank data. Conclusion: A new gene from the healthy Chinese was found, and it was named and accepted by GenBank.