HLA类基因PCR-SSP分型技术在骨髓移植配型中的应用

HLA-Ⅱ genotyping by PCR-SSP technique in application of bone marron transplantation

目的建立快速、准确的 HL A基因分型方法 ,满足临床移植配型的需要。方法通过序列特异性引物聚合酶链反应 (PCR- SSP) ,对拟行骨髓移植的 10例血液病患者及 12名相关供者的 HL A- 类基因 DRB1,DQB1位点扩增 ,琼脂糖凝胶电泳分析 PCR产物并确定其基因型。结果发现 2例患者与其相关供者 HL A- DRB1,DQB1基因全相同 ,其中 1例成功进行了骨髓移植。结论该方法具有快速准确、特异性高、简便省时的特点。

ObjectiveTo establish a rapid,accurate HLA DNA typing method in application of BMT.MethodsWith the use of polymerase chain reaction sequence specific primers(PCR SSP).We amplified the DRB1 and DQB1 loci of 10 patients planning to take BMT and 12 potential related donors,and identified their genotype by analysis of amplification products with agarose gel electrophoresis.Result Among these patients,2 cases matched with their donors and one took BMT successfully.ConclusionsThis method is rapid,accurate,high specific and convenient. [