正常人肾上腺组织基因表达谱的研究

Investigation of the profilings of the gene expression in normal human adrenal tissue

目的 建立正常人肾上腺组织基因表达目录。方法 肾上腺文库构建、大规模表达序列标签 (ESTs)测序及生物信息学分析。结果 基因 /蛋白表达类的基因在肾上腺组织表达最高 ,代谢类基因次之。前 3位高表达基因均为类固醇激素合成酶类及相关蛋白。肾上腺表达CRH、尿皮素(CRH相关蛋白 )、黑色素浓集素 (MCH)、可卡因和安菲他明调节转录物 (CART)及垂体腺苷酸环化酶活化多肽 (PACAP)等激素及各类受体 ,包括细胞因子、神经肽及神经递质受体。还表达有低密度脂蛋白 (LDL)受体、高密度脂蛋白 (HDL)结合蛋白、胆固醇合成酶及多种载脂蛋白。结论 肾上腺最高表达的基因与其功能的特异性有关 ;肾上腺不仅是类固醇激素 ,亦是许多激素及细胞因子的合成场所 ;体内许多系统 ,如神经、免疫及造血等系统与肾上腺功能均有广泛的联系 ;肾上腺是合成胆固醇与载脂蛋白的重要场所 ;基因表达谱的研究提示肾上腺内可能存在CRH ACTH 皮质醇的局部调节系统。

Objective To establish the gene expression profiles in human normal adrenal glands (AD). Methods cDNA libraries of human normal adrenal gonads were constructed. Large scale sequencing and bioinformatics were used. Results The category of genes highly expressed in AD was gene/protein expression, followed by metabolism. The first three genes highly expressed in AD were the enzymes and protein involved in the biosynthesis of steroids. Hormones or secretory proteins, such as CRH, MCH, urocortin, CART (cocaine and amphetamine regulated transcript) and PACAP (pituitary adenylate cyclase activating polypeptide) were expressed in AD. Receptors of cytokines, neuropeptides and neurotransmitters were also expressed in AD. The synthases of steroids, low density lipoprotein receptor, high density lipoprotein binding protein and cholesterol were expressed in AD. Conclusions The genes highly expressed in AD are closely related to the specific functions of AD. Besides steroids, many peptide hormones and cytokines are also expressed in AD. The other systems in human body are extensively associated with AD by the receptors. It is suggested that there may be a local CRH ACTH cortisol regulatory network in AD.