一氧化氮在人工晶体植入术后眼内炎症反应中的作用

Effect of nitric oxide in ocular inflammatory response after intraocular lens implantation

目的 :探讨一氧化氮 (NO)在人工晶体植入术后眼内炎症反应中的作用。方法 :将新西兰白兔随机分成 3组 :(1)对照组 ;(2 )L -精氨酸 (L -Arg)组 ;(3)N -硝基 -L -精氨酸 (L -NNA)组。各组动物均施行晶体囊外摘除术 (ECCE) +人工晶体囊袋内植入术 (IOL) ,并于术后 0、1、3、7、14、30d观察术后眼内炎症反应 ,包括检查角膜水肿和前房渗出、房水细胞计数和分类 ;同时测定房水NO-2 /NO-3 含量。结果 :L -Arg组前房渗出、房水细胞总数和NO-2 /NO-3 含量均高于对照组 ;而L -NNA组前房渗出、房水细胞总数和房水NO-2 /NO-3 含量均低于对照组。结论 :NO在人体晶体植入术后眼内炎症反应中起一定的作用 ;使用NOS抑制剂可减少NO产生 ,降低术后眼内炎症反应。

AIM: To investigate the effect of nitric oxide (NO) in ocular inflammation after intraocular lens implantation. METHODS: All New Zealand rabbits were divided randomly into three groups: 1. control group, 2. L-arginine (L-Arg) group, 3. N-nitro-L-arginine (L-NNA) group. Extracapsular cataract extraction (ECCE) and posterior chamber intraocular lens implantation (IOL) were operated in all animals of each groups. The inflammatory response of all rabbit eyes, including cornea edema and anterior chamber exudation were investigated 1, 3, 7, 14, 30 days afteroperation. Meanwhile, aqueous humor was drawn for white blood cell (WBC) counting and classifying, as well as for NO- 2/NO- 3 measurement. RESULTS:NO- 2/NO- 3 contents, total WBC and anterior chamber exudation in aqueous humor of L-Arg group were higher than that in control group. While that of L-NNA group were lower than that in control group.CONCLUSION:NO plays a role in intraocular inflammatory response after intraocular lens implantation. L-NNA, a nitric oxide synthase exhibitor, decreased NO contents, therefore it may reduce intraocular inflammatory response after intraocular lens implantation.