人补体C_(5b～9)复合物对大鼠肾系膜细胞一氧化氮生成的影响

The effect of human complement C_(5b～9) complex on nitric oxide synthesis in glomerular mesangial cells of rats

目的 :探讨人补体C5b～ 9复合物对大鼠肾小球系膜细胞 (MC)合成一氧化氮 (NO)的影响。方法 :首先提取人补体C5b～ 9复合物 ,同时培养出大鼠肾小球MC。然后用C5b～ 9复合物刺激MC ,观察刺激后 6h、2 4h、和 48h培养上清液中NO代谢产物 -硝酸根 (NO-3 )和亚硝酸根 (NO 2 )量的变化 ,并测定刺激后 2 4h、48h时MC内环鸟苷酸 (cGMP)的水平。结果 :用人C5b～ 9复合物刺激大鼠MC后可致培养上清中NO 3 /NO 2 含量增加 ,细胞内cGMP水平上升。NO合成增多能够被NO合酶 (NOS)抑制剂—─ -NG-硝基 -L -精氨酸甲基酯 (L -NAME)所抑制。结论 :人C5b～ 9复合物能够增加大鼠肾MC的NO合成。

AIM:To study the effect of human complement C 5b～9 complex on nitric oxide(NO) synthesis of glomerular mesangial cells (MC). METHODS: First, the human complement C 5b～9 complexes were isolated and glomerular MC of rats were cultured. Second, the MC were stimulated with C 5b～9 complex and changes of metabolism products of NO(NO 3 and NO 2) in MC culture supernatant at 6,24 and 48 hours after C 5b～9 stimulating were detected. Moreover, cGMP levels in cultured MC were also measured. RESULTS: NO 3/NO 2 contents from culture supernatant and cGMP levels in MC were increased parallelly after C 5b～9 complex stimulation. Further, NO synthesis was inhibited by L-NG-nitro-arginine-methylester(L-NAME). CONCLUSION: NO synthesis of rat glomerular MC was incerased by human complement C 5b～9 stimulation.