硒对CCl_4诱导大鼠肝细胞和Ito细胞外基质产生的影响

Inhibition Roles of Selenium on Extracellular Matrix of Hepatocytes and Ito Cells Injured by Carbon Tetrachloride

采用原位灌注方法分离大鼠肝细胞和储脂细胞 (Ito细胞 ) ,观察微量元素硒 (Se)对CCl4 作用下大鼠肝细胞、Ito细胞细胞外基质 (ECM)产生的拮抗作用。实验结果表明 :Se主要通过提高CCl4 作用下肝细胞、Ito细胞谷胱甘肽过氧化物酶(GPx)活性 ,阻止脂质过氧化损伤 ,继而抑制两种细胞Ⅲ型前胶原 (PCⅢ )

To study the inhibitory effects of Selenium(Se) on extracellular matrix(ECM)production of hepatocytes and ito cells in vitro,rat hepatocytes and ito cells were isolated and cultured.The results showed that addition of carbon tetrachloride to hepatocytes and ito cells pretreated with 0.36nmol/L Se 4 hours before,the incorporation of 3H proline and the secretion of precollagen type Ⅲ and the production of malondialdehyde were markedly inhibited and the activity of glutathione peroxidase(GPx)obviously increased.Se also inhibited the excretion of hyaluronic acid、laminin and fibronectin in cultured ito cells.The analysis of correlation indicated that the effects of Se on antioxidative injury and ECMs production depended mainly on increasing the activity of GPx.