过量表达辣椒CaNPR1提高烟草对青枯菌的抗性

Overexpression of CaNPR1 Enhances Resistance to Ralstonia solanacearum Infection in Tobacco

对水稻和拟南芥等模式植物的研究表明,NPR1(nonexpressor of pathogenesis-related genes 1)是依赖于SA通路的防御反应调节基因,但在辣椒和烟草等茄科作物中该蛋白的功能还鲜有报道。研究从辣椒cDNA文库中分离获得一个NPR1的类似物全长cDNA(CaNPR1),并获得了其超表达的转基因烟草T1代株系。研究结果表明,这些株系与其野生型植株没有明显表型差异,但却表现出较野生型植株更高的抗青枯菌侵染活性。同时,研究还发现CaNPR1的超表达还显著提高了防御相关基因的表达,表明NPR1在不同植物间具有较强的功能保守性。

The NPR1 （ nonexpressor of pathogenesis-related genes 1 ） gene plays an impor- tant role in SA-depended defense response processes in model plants such as Arabidopsis and rice. However,the function and underlying mechanism of this gene in non-model plants, in- cluding pepper （Capsicum annuum） and other Solanaceae, remain poorly understood. Here, we report on the functional characterization of CaNPR1 from pepper. Our data showed that the full-length cDNA of CaNPR1 was isolated and overexpression of CaNPR1 was construed in to- bacco K326. Compared to wild type K326, CaNPRI-OE lines did not exhibit any phenotypic differences, but exhibited higher resistance to Ralstonia solanacearum inoculation. In addition, this was coupled with enhanced transcript levels of defense-related marker genes, suggesting that CaNPR1 possessed higher conservative function in cross species.