摘要
目的观察Nogo-p4对大鼠脊髓来源神经干细胞(NSCs)分化形成双极形星形胶质细胞突起长度的影响。方法取4只出生24h内的Wistar大鼠,悬浮培养法培养大鼠脊髓来源的NSCs,形成神经球后,以免疫荧光法鉴定NSCs。把NSCs分为A、B两组,A组加入血清,B组加入血清和Nogo-p4,分化7d后,胶质纤维酸性蛋白(GFAP)抗体标记星形胶质细胞,测量双极形星形胶质细胞突起长度。结果 NSCs分化第7天,A组和B组均可形成双极形星形胶质细胞。B组中双极形星形胶质细胞的突起长度明显短于A组。结论 Nogo-p4可以显著抑制脊髓来源NSCs分化成的双极形星形胶质细胞的突起生长。
Objective To observe the effect of Nogo-p4 on process growth of bipolar astrocyte differentiated from neural stem cells （NSCs）. Methods Neurospheres were collected by suspension culture of cells obtained from spinal cords of 4 Wistar rats born in 24 hours. Immunofluorescence method was used to identify NSCs and detect expression of glial fibrillary acidic portein （GFAP） after NSCs differentiated. The NSCs were divided into groups A and B. FBS was added to group A and FBS ＋ Nogo-p4 was added to group B to initiate glial differentiation. The process length of bipolar astrocytes was measured. Results Seven days after differentiation, bipolar astrocytes were observed in both groups. The length of the process on bipolar astrocyte in group B was shorter than that in group A significantly. Conclusion The process growth of bipolar astrocytes differentiated from NSCs derived from the spinal cord of rats is inhibited significantly by Nogo-p4.
出处
《解剖学报》
CAS
CSCD
北大核心
2012年第6期730-733,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(81070971/H0910)
辽宁省教育厅科技研究资助项目(L2010621)
