建立卵泡刺激素受体单位点基因突变体的卵巢癌SKOV3细胞和裸鼠模型

Ovarian carcinoma SKOV3 cell line and nude mice model with transfecting unit point mutant gene in follicle stimulating hormone receptor

目的建立转染卵泡刺激素受体(FSHR)307和680位点突变的上皮性卵巢癌SKOV3细胞系和裸鼠动物模型。方法将人卵巢颗粒细胞(来自体外受精取卵时废弃的颗粒细胞),行原代培养后用于FSHR RNA提取;FSHR和FSHR307、680位点突变重组蛋白构建;将FSHR和FSHR307、680位点突变蛋白转染SKOV3细胞株;将处理后的3组SKOV3细胞接种到裸鼠皮下,8周后处死裸鼠,并取瘤组织验证。结果建立了307和680位点突变的FSH受体高表达的上皮性卵巢癌SKOV3细胞系和裸鼠动物模型。结论成功地建立了307和680位点突变的FSH受体高表达的SKOV3上皮性卵巢癌细胞系和裸鼠移植瘤模型,为FSH在上皮性卵巢癌中的作用和治疗提供了一种新的细胞模型和动物模型。

Objective To establish an epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site in order to provide two experimental models for further study and treatment of epithelial ovarian carcinoma by follicle stimulating hormome（FSH）. Methods FSHR RNA from the primary culture of granular cells in human ovary were extracted. Recombination protein about FSHR and mutant point in FSHR 307 and 680 site were constructed and transfeeted to SKOV3 cell line. SKOV3, SKOV3 cells with FSHR and mutant point in FSHR 307 and 680 site cells were inoculated subcutaneously in female nude mice to form solid tumors. Pathological examinations were performed after 8 weeks. Results An epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site were established. Conclusion Establishing an epithelial ovarian carcinoma with transfecting mutant gene in FSHR 307 and 680 site in vitro and in vivo offers two considerable models to explore and treat epithelial ovarian carcinoma by FSH.