海洛因戒断复吸对大鼠睾丸表皮生长因子、嗜铬粒素A表达及血清卵泡刺激素与黄体生成素的影响

Influence of heroin withdrawal and relapse on testicular epidermal growth factor,chromogranin A immunoreactive cells and serum FSH,LH level in rats

目的探讨海洛因戒断、复吸对大鼠睾丸表皮生长因子(EGF)、嗜铬粒素A(CgA)免疫反应(IR)细胞、血清卵泡刺激素(FSH)与黄体生成素(LH)的影响。方法正常雄性SD大鼠63只,随机分为实验组21只、盐水对照组21只和正常对照组21只,实验组大鼠又分戒断组、脱毒组和复吸组。取正常对照组、盐水对照组及实验各组大鼠的睾丸组织和血清,采用免疫组织化学SABC法、图像分析法及放射免疫法进行研究。结果戒断组中EGF-IR细胞染色加深,免疫反应强度增强,平均灰度值降低(P<0.05);脱毒组EGF-IR细胞免疫反应强度有所下降,但仍强于正常及盐水对照组(P<0.05);复吸组EGF-IR细胞免疫反应强度减弱,平均灰度值升高(P<0.05)。戒断组CgA-IR细胞免疫反应强度减弱,平均灰度值升高(P<0.05);脱毒组CgA-IR细胞免疫反应强度在脱毒后逐渐恢复;复吸组CgA-IR细胞染色变浅,平均灰度值升高(P<0.05)。放射免疫测定结果表明,LH在大鼠戒断和复吸期间处于较低水平(P<0.05),脱毒期间与正常组和盐水组比较无明显差异;FSH在大鼠戒断、脱毒、复吸期间变化差异无统计学意义。结论海洛因戒断、复吸期间大鼠睾丸EGF-、CgA-IR细胞的分泌及血清LH、FSH水平发生改变,参与了机体的内分泌调节。

Objective To study the changes of testicular epidermal growth factor （ EGF）, chromogranin A （CgA） immunoreactive（IR） cells, and the changes of serum follicle-stimulating hormone（FSH） , luteinizing hormone（LH） level in rats during heroin withdrawal, methadone detoxification and heroin relapse, in order to investigate the mechanisms. Methods Sixty-three adult male SD rats were randomly divided into normal control group （NCG）, saline control group （SCG） ,and experiment group （EG）. The rats in the EG group were divided into heroin withdrawal group （HWG）, methadone detoxication group （MDG） and heroin relapse group （HRG）. Serum was obtained via heart puncture, and the testis was excised soon after heart puncture. Immunohistochemical SABC method, image analysis, and radioimmunoassay were used. Results Compared with NCG and SCG, the mean gray value of EGF-IR cells drawdown in HWG, and revived after the treatment of methadone but still at a high secretion state. In HRG the mean gray value of EGF-IR cells was increased. Compared with NCG and SCG, the mean gray value of CgA-IR cells was declined in HWG, which restored after methadone disposal. In HRG, the immune staining intensity of CgA-IR cells declined. Serum LH levels in HWG and HRG were at a low level, but FSH had no statistically significant in the whole process. Conclusion The testis secretion of EGF,CgA and serum LH level was changed during heroin withdrawal and relapse. The results suggest that the testis secretion of EGF, CgA and serum LH may participate in the process of body adjusting during heroin withdrawal, methadonedetoxication and heroin relapse period.