豹皮樟总黄酮调控瘦素、TGF-β1对肝纤维化大鼠的预防作用研究

Regulatory effects of Litsea Coreana level on the expressions of Leptin and TGF-β1 to prevent the hepatic fibrosis in rats

目的研究豹皮樟总黄酮(TFLC)调控瘦素(leptin)和转化生长因子-β1(TGF-β1)对肝纤维化大鼠的预防作用。方法采用CCl4诱导大鼠肝纤维化模型,TFLC灌胃给药第12周末,检测ALT、AST及HA、LN、PⅢNP、CⅣ含量;检测Ⅰ型胶原(CollagenⅠ)、瘦素、TGF-β1的含量;检测Hyp及组织病理变化;检测瘦素受体(OB-Rb)、TGFβ1Ⅰ型受体(TGFβR1)、Smad3 mRNA及蛋白表达。结果 TFLC(200、400 g.L-1)能明显降低肝纤维化大鼠肝纤维化指标及血清Ⅰ型胶原、瘦素、TGF-β1水平;降低Ob-Rb、TGFβR1、Smad3的mRNA及蛋白表达。结论 TFLC能有效预防CCl4诱导的大鼠肝纤维化,可能与下调肝内瘦素及其受体,抑制TGF-β1/Smad通路有关。

Aim To evaluate whether Litsea Coreana level（TFLC） would have regulatory effects on the expressions of Leptin and TGF-β1 of hepatic fibrosis in rats.Methods The liver fibrosis model rats were in duced by injecting 50% CCl 4（1 ml.kg-1） twice a week for 12 weeks.From the first week,all the therapeutic groups were treated with TFLC（100,200,400 mg.kg-1） and the colchicines（0.1 mg.kg-1） respectively.At the end of the twelfth week,the levels of ALT,AST,HA,LN,P Ⅲ NP,CIV,Collagen Ι,Leptin,TGF-β1 in serum and Hyp in liver were measured.Masson staining was used to evaluate the degree of hepatic fibrosis.The mRNA and protein expressions of Ob-Rb,TGF-βR1 and Smad3 in liver were detected by RT-PCR and Western blot.Results TFLC（200 and 400 mg.kg-1） treatment significantly reduced the elevations of ALT,AST,HA,LN,PⅢNP,CIV,Collagen Ⅰ,Leptin,TGF-β1 in serum and Hyp in liver.In addition,TFLC treatment improved the morphologic changes of hepatic fibrosis,suppressed expressions of Ob-Rb,TGF-βR1,Smad3 in the liver fibrosis in rats.Conclusions TFLC is able to ameliorate liver injury and protect rats from liver fibrosis.This process may be related to inhibiting the expression of Ob-Rb,TGFβR1 and Smad3.