转基因抗虫棉棉籽组分的代谢组学研究

Metabonomic Study on the Composition of Insect-resistant Transgenic Cottonseeds

采用核磁共振氢谱(1H NMR)获取不同转基因抗虫棉棉籽及其非转基因对照棉籽的代谢物谱,并结合多维统计分析探查植物基因工程操作是否引发棉籽中代谢产物种类和数量的非预期改变。结果表明,不同棉籽的1H NMR谱图轮廓基本特征相似,均可分为氨基酸区、碳水化合物区及芳香区;当谱图按分区扩展后,多处峰信号在转基因与非转基因对照间有明显的不同。采用偏最小二乘法-判别分析(PLS-DA)可实现转基因抗虫棉棉籽与非转基因对照的代谢轮廓判别且分类的效果优于主成分分析(PCA)方法。转基因抗虫棉籽与其非转基因对照间的主要差异代谢物与初级氮代谢、三羧酸循环及脂肪酸代谢有关。

The term of metabolome has been used to describe the responses of plant to external perturbations recently. The study of metabolite profiling of different insect-resistant cottonseeds and their non-transgeniccounterparts using metabolic variations associated with genetic modifications. The results obtained showed that the overall appearance of the spectrum was quite similar among different cottonseeds. However, there were many different peak signals in the animo acids region （3 - 0. 5 ppm） of the expansion spectra of transgenic cottonseeds when compared with their controls. Ahhough score plots generated using principal component analysis showed the potential to distinguish transgenic cottonseeds from non-transgenic controls, a better classification between them was obtained by partial least square-discriminant analysis. The major compounds contributing to the discrimination were those metabolites that involved the metabolic pathway of fatty acid, the primary nitrogen metabolism and the tricarboxylic acid cycle.