气相色谱法分离测定环孢素A中乙醇及丙二醇的含量

Determination of Ethanol and Propylene Glycol in Cyclosprin A by Gas Chromatography

目的 :建立一个气相色谱条件同时分离测定环孢素A中乙醇及丙二醇的含量。方法 :以GDX - 10 1为固定相 ,柱长为 2m ,进样口温度为 2 10℃ ,检测器为 2 80℃ ,柱温采用程序升温 ,氮气为载气 ,以二甲基亚砜为溶剂 ,以正丙醇为内标。结果 :乙醇及丙二醇进样量分别在 2 0～ 6 0 μg ,1 0～ 3 0 μg ,其峰面积与浓度呈良好的线性关系 ,加样回收率分别为 99 9% (RSD <0 8% ,n =5 ) ,10 1 4 % (RSD <1 1% ,n =5 ) ,精密度良好。结论 :此气相色谱条件可同时测定环孢素A中乙醇及丙二醇的含量 。

Objective:This paper reports a GC method for determination ethanol and propylene glycol in cyclosprin A. Methods:Using as a column packed with the GDX-101 as stationary phase, The column length is 2 m. The injector temperature is 210 ℃.The detector temperature is 280 ℃. The column temperature rise by programing. The carrie gas is Nitrogen. The solvent was dimethyl sulfoxide. The internal standard is n -propanol. Results:The standard curves are linear for ethanol within 2 0～6 0 μg, for propylene glycol 1 0～3 0 μg. The average recoveries are 99 9%( RSD <0 8%, n =5) for ethanol, 101 4%( RSD <1 1%, n=5 )for propylene glycol, respectively. Conclusion:The results showed this method is simple,rapid and precision.