微乳薄层色谱用于黄酮类成分分离鉴定的研究

Studies on Separation and Identification of Flavonoid Compositions with Microemulsion Thin Layer Chromatography

目的 :探讨微乳薄层色谱法在分离鉴定黄酮类成分中的应用。方法 :以 6种SDS -正丁醇 -正庚烷 -水微乳液作为展开剂 ,通过聚酰胺薄层层析 ,分离和检测 14种中药材、饮片及中成药的黄酮类成分 ,考察了微乳液类型、酸度等因素对分离效果的影响。结果 :含水量 75 % (O/W型 )微乳液 -甲酸 ( 9 5∶0 5 )为适宜的展开剂。与常用展开剂比较 ,分离效果及检测灵敏度显著提高 ,且斑点圆而集中 ,Rf值高的组分亦无扩散现象。结论 :建立了一种新型的色谱技术 ,可以简便、准确、高效地分离和鉴定芦丁、槲皮素。

Objective:To investigate the use of microemulsion TLC on the separation and identification of flavonoid drugs.Methods:Fourteen kinds of flavonoid drugs were separated and identified by six kinds of SDS/ n -C 4H 9 OH/ n -C 7H 16 /H 2O microemulsions as mobile phase on polyamide film TLC.Affection on separation by the kinds of microemulsions and acidity were investigated.Results:The best mobile phase was the O/W microemulsion(containing 75% water)-formic acid(9 5∶0 5).Compared with general mobile phases, the spot effects of microemulsion mobile phase could be improved.Conclusion:This new method is simple,accurate and efficient for the separation and identification of flavonoid drugs.